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u0750 100g  (New England Biolabs)


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    New England Biolabs u0750 100g
    U0750 100g, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 6297 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/u0750+100g/Gibson+Assembly+Master+Mix/pm36853680-37-211-237
    Average 99 stars, based on 6297 article reviews
    u0750 100g - by Bioz Stars, 2026-10
    99/100 stars

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    Virus:

    Article Title: Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-HA ABLab Cat# 12CA5 Rabbit polyclonal anti-Pgk1 Acris Cat# AP21371AF-N IRDye 800CW goat anti-mouse IgG LI-COR Cat# 926-32210 IRDye 800CW goat anti-rabbit IgG LI-COR Cat# 926-32211 Bacterial and virus strains NEB5a competent E. coli NEB Cat# C2987I Chemicals, peptides, and recombinant proteins Adenine hemisulfate salt Sigma-Aldrich Cat# A9126-100G 13C6 Arg-6 Silantes Cat# 201204102 13C615N4 Arg-10 Silantes Cat# 201604102 13C615N2 Lys-8 Silantes Cat# 211604102 D4 Lys-4 Silantes Cat# 211104113 Dextrose/D-Glucose Fisher Chemical Cat# D16-10 Difco Yeast Nitrogen Base without Amino Acids (YNB) BD Life Sciences Cat# 291940 D-(+)-Galactose Bio Basic Cat# GB0215 IGEPAL CA-630 (NP-40) Sigma-Aldrich Cat# I3021-100ML L-Histidine monohydrochloride monohydrate Sigma-Aldrich Cat# H8125-100G L-Leucine Sigma-Aldrich Cat# L8000-100G L-Lysine monohydrochloride Sigma-Aldrich Cat# L5626-100G L-Methionine Sigma-Aldrich Cat# M9625-100G L-Tryptophan Sigma-Aldrich Cat# T0254-100G Phenylmethylsulfonyl fluoride (PMSF) Roche Cat# 1359061 cOmplete , EDTA-free Protease Inhibitor Cocktail (PIC) Roche Cat# 5056489001 Raffinose, pentahydrate, high purity Bio Basic Cat# RJ392 Sequencing grade modified Trypsin, frozen Promega Cat# V5113 Sera-Mag SpeedBeads A (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophilic) Thermo Scientific Cat# 09-981-121 Sera-Mag SpeedBeads B (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophobic) Thermo Scientific Cat# 09-981-123 Sodium carbonate ReagentPlus , R99.5% Sigma-Aldrich Cat# S2127-500G Synthetic Dropout medium for yeast without Lysine Sigma-Aldrich Cat# Y1896-20G Uracil Sigma-Aldrich Cat# U0750-100G (Continued on next page) 2 STAR Protocols 4, 102059, March 17, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Gibson Assembly Master mix NEB Cat# E2611L Q5 high-fidelity DNA polymerase NEB Cat # M0491L Deposited data Mass spectrometry results of pellet and total cell lysate fractions from single and double pulse SILAC (Exp1 and Exp3) 1 PRoteomics IDEntification (PRIDE) repository PXD024336 Experimental models: Organisms/strains BY4741 strains (MATa, his3D1, leu2D0, met15D0, ura3D0) Hieter Lab YTM408 SILAC strain (MATa, his3D1, leu2D0, ura3D0, MET15, arg4D::KanMX6, lys2D0) Measday Lab YTM1173 Recombinant DNA pRS426-GAL1p-3HA-CYC1t Mayor Lab BPM1809 Software and algorithms Bruker Compass HyStar 5.0 SR1 Bruker Daltonics Ver.

    Recombinant:

    Article Title: Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-HA ABLab Cat# 12CA5 Rabbit polyclonal anti-Pgk1 Acris Cat# AP21371AF-N IRDye 800CW goat anti-mouse IgG LI-COR Cat# 926-32210 IRDye 800CW goat anti-rabbit IgG LI-COR Cat# 926-32211 Bacterial and virus strains NEB5a competent E. coli NEB Cat# C2987I Chemicals, peptides, and recombinant proteins Adenine hemisulfate salt Sigma-Aldrich Cat# A9126-100G 13C6 Arg-6 Silantes Cat# 201204102 13C615N4 Arg-10 Silantes Cat# 201604102 13C615N2 Lys-8 Silantes Cat# 211604102 D4 Lys-4 Silantes Cat# 211104113 Dextrose/D-Glucose Fisher Chemical Cat# D16-10 Difco Yeast Nitrogen Base without Amino Acids (YNB) BD Life Sciences Cat# 291940 D-(+)-Galactose Bio Basic Cat# GB0215 IGEPAL CA-630 (NP-40) Sigma-Aldrich Cat# I3021-100ML L-Histidine monohydrochloride monohydrate Sigma-Aldrich Cat# H8125-100G L-Leucine Sigma-Aldrich Cat# L8000-100G L-Lysine monohydrochloride Sigma-Aldrich Cat# L5626-100G L-Methionine Sigma-Aldrich Cat# M9625-100G L-Tryptophan Sigma-Aldrich Cat# T0254-100G Phenylmethylsulfonyl fluoride (PMSF) Roche Cat# 1359061 cOmplete , EDTA-free Protease Inhibitor Cocktail (PIC) Roche Cat# 5056489001 Raffinose, pentahydrate, high purity Bio Basic Cat# RJ392 Sequencing grade modified Trypsin, frozen Promega Cat# V5113 Sera-Mag SpeedBeads A (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophilic) Thermo Scientific Cat# 09-981-121 Sera-Mag SpeedBeads B (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophobic) Thermo Scientific Cat# 09-981-123 Sodium carbonate ReagentPlus , R99.5% Sigma-Aldrich Cat# S2127-500G Synthetic Dropout medium for yeast without Lysine Sigma-Aldrich Cat# Y1896-20G Uracil Sigma-Aldrich Cat# U0750-100G (Continued on next page) 2 STAR Protocols 4, 102059, March 17, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Gibson Assembly Master mix NEB Cat# E2611L Q5 high-fidelity DNA polymerase NEB Cat # M0491L Deposited data Mass spectrometry results of pellet and total cell lysate fractions from single and double pulse SILAC (Exp1 and Exp3) 1 PRoteomics IDEntification (PRIDE) repository PXD024336 Experimental models: Organisms/strains BY4741 strains (MATa, his3D1, leu2D0, met15D0, ura3D0) Hieter Lab YTM408 SILAC strain (MATa, his3D1, leu2D0, ura3D0, MET15, arg4D::KanMX6, lys2D0) Measday Lab YTM1173 Recombinant DNA pRS426-GAL1p-3HA-CYC1t Mayor Lab BPM1809 Software and algorithms Bruker Compass HyStar 5.0 SR1 Bruker Daltonics Ver.

    Protease Inhibitor:

    Article Title: Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-HA ABLab Cat# 12CA5 Rabbit polyclonal anti-Pgk1 Acris Cat# AP21371AF-N IRDye 800CW goat anti-mouse IgG LI-COR Cat# 926-32210 IRDye 800CW goat anti-rabbit IgG LI-COR Cat# 926-32211 Bacterial and virus strains NEB5a competent E. coli NEB Cat# C2987I Chemicals, peptides, and recombinant proteins Adenine hemisulfate salt Sigma-Aldrich Cat# A9126-100G 13C6 Arg-6 Silantes Cat# 201204102 13C615N4 Arg-10 Silantes Cat# 201604102 13C615N2 Lys-8 Silantes Cat# 211604102 D4 Lys-4 Silantes Cat# 211104113 Dextrose/D-Glucose Fisher Chemical Cat# D16-10 Difco Yeast Nitrogen Base without Amino Acids (YNB) BD Life Sciences Cat# 291940 D-(+)-Galactose Bio Basic Cat# GB0215 IGEPAL CA-630 (NP-40) Sigma-Aldrich Cat# I3021-100ML L-Histidine monohydrochloride monohydrate Sigma-Aldrich Cat# H8125-100G L-Leucine Sigma-Aldrich Cat# L8000-100G L-Lysine monohydrochloride Sigma-Aldrich Cat# L5626-100G L-Methionine Sigma-Aldrich Cat# M9625-100G L-Tryptophan Sigma-Aldrich Cat# T0254-100G Phenylmethylsulfonyl fluoride (PMSF) Roche Cat# 1359061 cOmplete , EDTA-free Protease Inhibitor Cocktail (PIC) Roche Cat# 5056489001 Raffinose, pentahydrate, high purity Bio Basic Cat# RJ392 Sequencing grade modified Trypsin, frozen Promega Cat# V5113 Sera-Mag SpeedBeads A (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophilic) Thermo Scientific Cat# 09-981-121 Sera-Mag SpeedBeads B (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophobic) Thermo Scientific Cat# 09-981-123 Sodium carbonate ReagentPlus , R99.5% Sigma-Aldrich Cat# S2127-500G Synthetic Dropout medium for yeast without Lysine Sigma-Aldrich Cat# Y1896-20G Uracil Sigma-Aldrich Cat# U0750-100G (Continued on next page) 2 STAR Protocols 4, 102059, March 17, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Gibson Assembly Master mix NEB Cat# E2611L Q5 high-fidelity DNA polymerase NEB Cat # M0491L Deposited data Mass spectrometry results of pellet and total cell lysate fractions from single and double pulse SILAC (Exp1 and Exp3) 1 PRoteomics IDEntification (PRIDE) repository PXD024336 Experimental models: Organisms/strains BY4741 strains (MATa, his3D1, leu2D0, met15D0, ura3D0) Hieter Lab YTM408 SILAC strain (MATa, his3D1, leu2D0, ura3D0, MET15, arg4D::KanMX6, lys2D0) Measday Lab YTM1173 Recombinant DNA pRS426-GAL1p-3HA-CYC1t Mayor Lab BPM1809 Software and algorithms Bruker Compass HyStar 5.0 SR1 Bruker Daltonics Ver.

    Sequencing:

    Article Title: Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-HA ABLab Cat# 12CA5 Rabbit polyclonal anti-Pgk1 Acris Cat# AP21371AF-N IRDye 800CW goat anti-mouse IgG LI-COR Cat# 926-32210 IRDye 800CW goat anti-rabbit IgG LI-COR Cat# 926-32211 Bacterial and virus strains NEB5a competent E. coli NEB Cat# C2987I Chemicals, peptides, and recombinant proteins Adenine hemisulfate salt Sigma-Aldrich Cat# A9126-100G 13C6 Arg-6 Silantes Cat# 201204102 13C615N4 Arg-10 Silantes Cat# 201604102 13C615N2 Lys-8 Silantes Cat# 211604102 D4 Lys-4 Silantes Cat# 211104113 Dextrose/D-Glucose Fisher Chemical Cat# D16-10 Difco Yeast Nitrogen Base without Amino Acids (YNB) BD Life Sciences Cat# 291940 D-(+)-Galactose Bio Basic Cat# GB0215 IGEPAL CA-630 (NP-40) Sigma-Aldrich Cat# I3021-100ML L-Histidine monohydrochloride monohydrate Sigma-Aldrich Cat# H8125-100G L-Leucine Sigma-Aldrich Cat# L8000-100G L-Lysine monohydrochloride Sigma-Aldrich Cat# L5626-100G L-Methionine Sigma-Aldrich Cat# M9625-100G L-Tryptophan Sigma-Aldrich Cat# T0254-100G Phenylmethylsulfonyl fluoride (PMSF) Roche Cat# 1359061 cOmplete , EDTA-free Protease Inhibitor Cocktail (PIC) Roche Cat# 5056489001 Raffinose, pentahydrate, high purity Bio Basic Cat# RJ392 Sequencing grade modified Trypsin, frozen Promega Cat# V5113 Sera-Mag SpeedBeads A (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophilic) Thermo Scientific Cat# 09-981-121 Sera-Mag SpeedBeads B (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophobic) Thermo Scientific Cat# 09-981-123 Sodium carbonate ReagentPlus , R99.5% Sigma-Aldrich Cat# S2127-500G Synthetic Dropout medium for yeast without Lysine Sigma-Aldrich Cat# Y1896-20G Uracil Sigma-Aldrich Cat# U0750-100G (Continued on next page) 2 STAR Protocols 4, 102059, March 17, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Gibson Assembly Master mix NEB Cat# E2611L Q5 high-fidelity DNA polymerase NEB Cat # M0491L Deposited data Mass spectrometry results of pellet and total cell lysate fractions from single and double pulse SILAC (Exp1 and Exp3) 1 PRoteomics IDEntification (PRIDE) repository PXD024336 Experimental models: Organisms/strains BY4741 strains (MATa, his3D1, leu2D0, met15D0, ura3D0) Hieter Lab YTM408 SILAC strain (MATa, his3D1, leu2D0, ura3D0, MET15, arg4D::KanMX6, lys2D0) Measday Lab YTM1173 Recombinant DNA pRS426-GAL1p-3HA-CYC1t Mayor Lab BPM1809 Software and algorithms Bruker Compass HyStar 5.0 SR1 Bruker Daltonics Ver.

    Modification:

    Article Title: Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-HA ABLab Cat# 12CA5 Rabbit polyclonal anti-Pgk1 Acris Cat# AP21371AF-N IRDye 800CW goat anti-mouse IgG LI-COR Cat# 926-32210 IRDye 800CW goat anti-rabbit IgG LI-COR Cat# 926-32211 Bacterial and virus strains NEB5a competent E. coli NEB Cat# C2987I Chemicals, peptides, and recombinant proteins Adenine hemisulfate salt Sigma-Aldrich Cat# A9126-100G 13C6 Arg-6 Silantes Cat# 201204102 13C615N4 Arg-10 Silantes Cat# 201604102 13C615N2 Lys-8 Silantes Cat# 211604102 D4 Lys-4 Silantes Cat# 211104113 Dextrose/D-Glucose Fisher Chemical Cat# D16-10 Difco Yeast Nitrogen Base without Amino Acids (YNB) BD Life Sciences Cat# 291940 D-(+)-Galactose Bio Basic Cat# GB0215 IGEPAL CA-630 (NP-40) Sigma-Aldrich Cat# I3021-100ML L-Histidine monohydrochloride monohydrate Sigma-Aldrich Cat# H8125-100G L-Leucine Sigma-Aldrich Cat# L8000-100G L-Lysine monohydrochloride Sigma-Aldrich Cat# L5626-100G L-Methionine Sigma-Aldrich Cat# M9625-100G L-Tryptophan Sigma-Aldrich Cat# T0254-100G Phenylmethylsulfonyl fluoride (PMSF) Roche Cat# 1359061 cOmplete , EDTA-free Protease Inhibitor Cocktail (PIC) Roche Cat# 5056489001 Raffinose, pentahydrate, high purity Bio Basic Cat# RJ392 Sequencing grade modified Trypsin, frozen Promega Cat# V5113 Sera-Mag SpeedBeads A (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophilic) Thermo Scientific Cat# 09-981-121 Sera-Mag SpeedBeads B (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophobic) Thermo Scientific Cat# 09-981-123 Sodium carbonate ReagentPlus , R99.5% Sigma-Aldrich Cat# S2127-500G Synthetic Dropout medium for yeast without Lysine Sigma-Aldrich Cat# Y1896-20G Uracil Sigma-Aldrich Cat# U0750-100G (Continued on next page) 2 STAR Protocols 4, 102059, March 17, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Gibson Assembly Master mix NEB Cat# E2611L Q5 high-fidelity DNA polymerase NEB Cat # M0491L Deposited data Mass spectrometry results of pellet and total cell lysate fractions from single and double pulse SILAC (Exp1 and Exp3) 1 PRoteomics IDEntification (PRIDE) repository PXD024336 Experimental models: Organisms/strains BY4741 strains (MATa, his3D1, leu2D0, met15D0, ura3D0) Hieter Lab YTM408 SILAC strain (MATa, his3D1, leu2D0, ura3D0, MET15, arg4D::KanMX6, lys2D0) Measday Lab YTM1173 Recombinant DNA pRS426-GAL1p-3HA-CYC1t Mayor Lab BPM1809 Software and algorithms Bruker Compass HyStar 5.0 SR1 Bruker Daltonics Ver.

    Magnetic Beads:

    Article Title: Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-HA ABLab Cat# 12CA5 Rabbit polyclonal anti-Pgk1 Acris Cat# AP21371AF-N IRDye 800CW goat anti-mouse IgG LI-COR Cat# 926-32210 IRDye 800CW goat anti-rabbit IgG LI-COR Cat# 926-32211 Bacterial and virus strains NEB5a competent E. coli NEB Cat# C2987I Chemicals, peptides, and recombinant proteins Adenine hemisulfate salt Sigma-Aldrich Cat# A9126-100G 13C6 Arg-6 Silantes Cat# 201204102 13C615N4 Arg-10 Silantes Cat# 201604102 13C615N2 Lys-8 Silantes Cat# 211604102 D4 Lys-4 Silantes Cat# 211104113 Dextrose/D-Glucose Fisher Chemical Cat# D16-10 Difco Yeast Nitrogen Base without Amino Acids (YNB) BD Life Sciences Cat# 291940 D-(+)-Galactose Bio Basic Cat# GB0215 IGEPAL CA-630 (NP-40) Sigma-Aldrich Cat# I3021-100ML L-Histidine monohydrochloride monohydrate Sigma-Aldrich Cat# H8125-100G L-Leucine Sigma-Aldrich Cat# L8000-100G L-Lysine monohydrochloride Sigma-Aldrich Cat# L5626-100G L-Methionine Sigma-Aldrich Cat# M9625-100G L-Tryptophan Sigma-Aldrich Cat# T0254-100G Phenylmethylsulfonyl fluoride (PMSF) Roche Cat# 1359061 cOmplete , EDTA-free Protease Inhibitor Cocktail (PIC) Roche Cat# 5056489001 Raffinose, pentahydrate, high purity Bio Basic Cat# RJ392 Sequencing grade modified Trypsin, frozen Promega Cat# V5113 Sera-Mag SpeedBeads A (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophilic) Thermo Scientific Cat# 09-981-121 Sera-Mag SpeedBeads B (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophobic) Thermo Scientific Cat# 09-981-123 Sodium carbonate ReagentPlus , R99.5% Sigma-Aldrich Cat# S2127-500G Synthetic Dropout medium for yeast without Lysine Sigma-Aldrich Cat# Y1896-20G Uracil Sigma-Aldrich Cat# U0750-100G (Continued on next page) 2 STAR Protocols 4, 102059, March 17, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Gibson Assembly Master mix NEB Cat# E2611L Q5 high-fidelity DNA polymerase NEB Cat # M0491L Deposited data Mass spectrometry results of pellet and total cell lysate fractions from single and double pulse SILAC (Exp1 and Exp3) 1 PRoteomics IDEntification (PRIDE) repository PXD024336 Experimental models: Organisms/strains BY4741 strains (MATa, his3D1, leu2D0, met15D0, ura3D0) Hieter Lab YTM408 SILAC strain (MATa, his3D1, leu2D0, ura3D0, MET15, arg4D::KanMX6, lys2D0) Measday Lab YTM1173 Recombinant DNA pRS426-GAL1p-3HA-CYC1t Mayor Lab BPM1809 Software and algorithms Bruker Compass HyStar 5.0 SR1 Bruker Daltonics Ver.

    Mass Spectrometry:

    Article Title: Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-HA ABLab Cat# 12CA5 Rabbit polyclonal anti-Pgk1 Acris Cat# AP21371AF-N IRDye 800CW goat anti-mouse IgG LI-COR Cat# 926-32210 IRDye 800CW goat anti-rabbit IgG LI-COR Cat# 926-32211 Bacterial and virus strains NEB5a competent E. coli NEB Cat# C2987I Chemicals, peptides, and recombinant proteins Adenine hemisulfate salt Sigma-Aldrich Cat# A9126-100G 13C6 Arg-6 Silantes Cat# 201204102 13C615N4 Arg-10 Silantes Cat# 201604102 13C615N2 Lys-8 Silantes Cat# 211604102 D4 Lys-4 Silantes Cat# 211104113 Dextrose/D-Glucose Fisher Chemical Cat# D16-10 Difco Yeast Nitrogen Base without Amino Acids (YNB) BD Life Sciences Cat# 291940 D-(+)-Galactose Bio Basic Cat# GB0215 IGEPAL CA-630 (NP-40) Sigma-Aldrich Cat# I3021-100ML L-Histidine monohydrochloride monohydrate Sigma-Aldrich Cat# H8125-100G L-Leucine Sigma-Aldrich Cat# L8000-100G L-Lysine monohydrochloride Sigma-Aldrich Cat# L5626-100G L-Methionine Sigma-Aldrich Cat# M9625-100G L-Tryptophan Sigma-Aldrich Cat# T0254-100G Phenylmethylsulfonyl fluoride (PMSF) Roche Cat# 1359061 cOmplete , EDTA-free Protease Inhibitor Cocktail (PIC) Roche Cat# 5056489001 Raffinose, pentahydrate, high purity Bio Basic Cat# RJ392 Sequencing grade modified Trypsin, frozen Promega Cat# V5113 Sera-Mag SpeedBeads A (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophilic) Thermo Scientific Cat# 09-981-121 Sera-Mag SpeedBeads B (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophobic) Thermo Scientific Cat# 09-981-123 Sodium carbonate ReagentPlus , R99.5% Sigma-Aldrich Cat# S2127-500G Synthetic Dropout medium for yeast without Lysine Sigma-Aldrich Cat# Y1896-20G Uracil Sigma-Aldrich Cat# U0750-100G (Continued on next page) 2 STAR Protocols 4, 102059, March 17, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Gibson Assembly Master mix NEB Cat# E2611L Q5 high-fidelity DNA polymerase NEB Cat # M0491L Deposited data Mass spectrometry results of pellet and total cell lysate fractions from single and double pulse SILAC (Exp1 and Exp3) 1 PRoteomics IDEntification (PRIDE) repository PXD024336 Experimental models: Organisms/strains BY4741 strains (MATa, his3D1, leu2D0, met15D0, ura3D0) Hieter Lab YTM408 SILAC strain (MATa, his3D1, leu2D0, ura3D0, MET15, arg4D::KanMX6, lys2D0) Measday Lab YTM1173 Recombinant DNA pRS426-GAL1p-3HA-CYC1t Mayor Lab BPM1809 Software and algorithms Bruker Compass HyStar 5.0 SR1 Bruker Daltonics Ver.

    Software:

    Article Title: Identification of newly translated thermo-sensitive proteins using pulse SILAC mass spectrometry and the GAL promoter system.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-HA ABLab Cat# 12CA5 Rabbit polyclonal anti-Pgk1 Acris Cat# AP21371AF-N IRDye 800CW goat anti-mouse IgG LI-COR Cat# 926-32210 IRDye 800CW goat anti-rabbit IgG LI-COR Cat# 926-32211 Bacterial and virus strains NEB5a competent E. coli NEB Cat# C2987I Chemicals, peptides, and recombinant proteins Adenine hemisulfate salt Sigma-Aldrich Cat# A9126-100G 13C6 Arg-6 Silantes Cat# 201204102 13C615N4 Arg-10 Silantes Cat# 201604102 13C615N2 Lys-8 Silantes Cat# 211604102 D4 Lys-4 Silantes Cat# 211104113 Dextrose/D-Glucose Fisher Chemical Cat# D16-10 Difco Yeast Nitrogen Base without Amino Acids (YNB) BD Life Sciences Cat# 291940 D-(+)-Galactose Bio Basic Cat# GB0215 IGEPAL CA-630 (NP-40) Sigma-Aldrich Cat# I3021-100ML L-Histidine monohydrochloride monohydrate Sigma-Aldrich Cat# H8125-100G L-Leucine Sigma-Aldrich Cat# L8000-100G L-Lysine monohydrochloride Sigma-Aldrich Cat# L5626-100G L-Methionine Sigma-Aldrich Cat# M9625-100G L-Tryptophan Sigma-Aldrich Cat# T0254-100G Phenylmethylsulfonyl fluoride (PMSF) Roche Cat# 1359061 cOmplete , EDTA-free Protease Inhibitor Cocktail (PIC) Roche Cat# 5056489001 Raffinose, pentahydrate, high purity Bio Basic Cat# RJ392 Sequencing grade modified Trypsin, frozen Promega Cat# V5113 Sera-Mag SpeedBeads A (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophilic) Thermo Scientific Cat# 09-981-121 Sera-Mag SpeedBeads B (GE Healthcare Sera-Mag SpeedBeads carboxyl magnetic beads, hydrophobic) Thermo Scientific Cat# 09-981-123 Sodium carbonate ReagentPlus , R99.5% Sigma-Aldrich Cat# S2127-500G Synthetic Dropout medium for yeast without Lysine Sigma-Aldrich Cat# Y1896-20G Uracil Sigma-Aldrich Cat# U0750-100G (Continued on next page) 2 STAR Protocols 4, 102059, March 17, 2023 Continued REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Gibson Assembly Master mix NEB Cat# E2611L Q5 high-fidelity DNA polymerase NEB Cat # M0491L Deposited data Mass spectrometry results of pellet and total cell lysate fractions from single and double pulse SILAC (Exp1 and Exp3) 1 PRoteomics IDEntification (PRIDE) repository PXD024336 Experimental models: Organisms/strains BY4741 strains (MATa, his3D1, leu2D0, met15D0, ura3D0) Hieter Lab YTM408 SILAC strain (MATa, his3D1, leu2D0, ura3D0, MET15, arg4D::KanMX6, lys2D0) Measday Lab YTM1173 Recombinant DNA pRS426-GAL1p-3HA-CYC1t Mayor Lab BPM1809 Software and algorithms Bruker Compass HyStar 5.0 SR1 Bruker Daltonics Ver.



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    KEY RESOURCES TABLE

    Journal: Current biology : CB

    Article Title: Critical roles of a RhoGEF-anillin module in septin architectural remodeling during cytokinesis

    doi: 10.1016/j.cub.2020.02.023

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Uracil , Sigma, Darmstadt, Germany , Cat #: U0750–100G.

    Techniques: Recombinant, Plasmid Preparation, Software